Cisplatin cytof
WebCisplatin uptake and effects on proliferation BxPC-3 pancreatic cancer cells and ME-180 cervical cancer cells exposed to cisplatin in vitro were pulse-labeled with 10 mM IdU for 30 min and analyzed for platinum and iodine content by mass cytometry. Figure 1a illustrates single cell measurements taken from control and cisplatin-treated cells ... Web[Optional] Cisplatin Viability Stain (if not performing cisplatin viability-stain, proceed to step #1 of the protocol below): Note: The following cisplatin-staining protocol has minimal …
Cisplatin cytof
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Web• Cell-ID™ Cisplatin (Cat# 201064) • Cell-ID™ Intercalator-103Rh (DVS Sciences Cat# 201103A [500µM] or 201103B [2000µM]) ... Leave cells pelleted until ready to run on CyTOF®. Immediately prior to CyTOF data acquisition, adjust cell concentration to 2.5-5 x 10. 5 /ml with MaxPar Water and filter cells into cell strainer cap tubes. http://cytoforum.stanford.edu/viewtopic.php?f=3&t=374
WebJul 25, 2024 · The cellular uptake of platinum-based chemotherapeutic agents (cisplatin, oxaliplatin, and carboplatin) after hyperthermia treatment was assessed using cytometry …
WebAug 8, 2010 · Cisplatin is one of the most effective anticancer agents widely used in the treatment of solid tumors. It is generally considered as a cytotoxic drug which kills cancer cells by damaging DNA and inhibiting DNA synthesis. How cells respond to cisplatin-induced DNA damage plays a critical role in deci … http://cytoforum.stanford.edu/viewtopic.php?f=4&t=216
WebMass Cytometry, also known as CyTOF (cytometry by time of flight), is similar to flow cytometry but uses heavy metals tagged antibodies instead of fluorophore tagged …
WebAug 17, 2015 · Doublet discrimination based on Ir191/193. Hello CyTOFers, Hopefully most of you are already aware of this, but I wanted to share a few gating examples illustrating some points of concern with traditional doublet discrimination based on Ir191/193+ DNA content and event length; namely that S-phase cells fall in the same region as true … t shirts missoulaWebJul 25, 2024 · The first platinum anticancer drug cisplatin is widely used to treat various types of cancers including ovarian, testicular, bladder, colorectal, lung, and head and neck cancers [ 16, 17 ]. Cisplatin crosslinks with purine bases on the DNA, interfering with DNA repair mechanisms, causing DNA damage and inducing apoptosis [ 16 ]. t-shirts mit firmenlogoWebApr 12, 2024 · (F and G) Shown are UMAPs visualizing CD8 + T cell populations in CyTOF datasets for non–small cell lung cancer (NSCLC) or LUAD (F) and GBM (G). ( H and I ) Shown is the network topology of SKCM (H) and GBM (I) scRNA-seq–based CellPhoneDB interaction predictions, filtered on the basis of TCGA tumor bulk RNA-seq data … t-shirts mit brusttascheWebSimilarly, short treatment of cells with the platinum-based reagent cisplatin is used in CyTOF to distinguish live from dead cells (25) (Figure 1A). ... View in full-text Context 7 … t shirts minecraftWebSep 10, 2015 · Cisplatin-labeling of antibody increases the analytical capacity of the CyTOF ® platform by two channels based on … t shirts mit coolen motivenWebFeb 3, 2024 · Imaging Mass Cytometry (IMC) is an expansion of mass cytometry, but rather than analyzing single cells in suspension, it uses laser ablation to generate plumes of particles that are carried to the mass cytometer by a stream of inert gas. Images reconstructed from tissue sections scanned by IMC have a resolution comparable to light … phil raffertyWebMay 12, 2024 · ClusterX: Fast clustering by automatic search and find of density peaks cytof_addToFCS: Add data to the original FCS files cytof_cluster: Subset detection by clustering cytof_clusterMtrx: Expression values by cluster cytof_clusterPlot: Scatter plot of the cluster results cytof_clusterStat: Statistics of the cluster results cytof_colorPlot: Plot … phil rainey