WebAug 1, 1972 · The better condition of cultivation for tetradecane 1,14-dicarboxylic acid (DC-16) production from n-hexadecane (n-C 16) by Candida cloacae MR-12 was … All strains used in this study are listed in Table 1. LB medium containing peptone (10 g/L), yeast extract (5 g/L), and NaCl (10 g/L), at pH 7.0 to 7.4, was used for the culture of E. coli DH5α. YPD medium containing glucose (20 g/L), peptone (20 g/L), and yeast extract powder (10 g/L) was used for the culture of C. … See more Based on the ctfat1p1 gene encoding the fatty acid transporter of C. tropicalis (NW_003020055.1), the upstream primer Fat1p F1 and the … See more After the single-knockout of the ctfat1p or ctpxa1p gene, the remaining copy was knocked out by constructing double-knockout fragment PFA6a-fat1p2 (5496 bp) or PFA6a … See more The preparation of C. tropicalis competent cells was performed according to the previously reported methods [17, 18]. ctfat1p1-kanr and ctpxa1p1-kanr were separately … See more Used C. tropicalis 1798 DNA as the template and Fat1p F2 and Fat1p R2 (Supplementary Table 1) as the primers for PCR amplification, a 3049-bp ctfat1p gene copy number was used to increase the ctfat1p gene … See more
Production of dodecanedioic acid via biotransformation of …
WebApr 11, 2024 · However, a potentially more valuable alternative is the production of long-chain dicarboxylic acids (LCDAs). This involves the use of aerobic yeasts, such as Candida tropicalis ATCC 20962. This organism is genetically modified to … WebJan 5, 2004 · A mutant of Candida tropicalis was used to convert n-alkanes to the corresponding α,ω-dicarboxylic acids (DC).Effects of medium pH on dioic acid … fish tank cloudy after adding new fish
Development of a promising microbial platform for the …
WebThe haploid strain identified as Candida sorbophila DS02 grew on glucose and dodecane, exhibiting greater cell shrinkage on the latter. In flask cultures with mixed alkanes (C10-16) and fatty acid methyl esters (C10-16), DS02 used mixed alkanes simultaneously unlike Candida tropicalis and Yarrowia lipolytica and showed high substrate resistance. WebJun 1, 2024 · Candida tropicalis can metabolize alkanes or fatty acids to produce long-chain dicarboxylic acids (DCAs). Fatty acid transporters located on the cell or peroxisome … WebMar 25, 2024 · Dicarboxylic acids are widely used in fine chemical and food industries as well as the monomer for polymerisation of high molecular material. Given the problems of environmental contamination and sustainable development faced by traditional production of dicarboxylic acids based on petrol, new approaches such as bio-based production … fish tank cloudy water