Webその結果、KOD -Plus- Neoを用いた場合でのみ、17.5kbまでの明瞭な増幅を確認することができました。. また、17.5kb以下の増幅においても、他社PCR酵素を大幅に上回る収量が認められました。. 3. 長鎖ターゲットの増幅. ヒトゲノムDNA (200 ng)をテンプレートに ... Weblarge insertions and deletions. The PCR fidelity of KOD-Plus- is greater than Taq DNA polymerase (ca. 80-fold); therefore, unexpected, 2nd-site mutations can be reduced. PCR reactions can be performed using standard PCR primers and do not require phosphorylated primers, because this protocol contains a ‘Phosphorylation Step’ of PCR products.
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