Then plvx-tetone-3xf-n-term puro
Splet06. nov. 2024 · SBI 第三代慢病毒载体 SBI offers thirdgeneration mostpopular HIV-1 based lentivector expression system which consists threemaincomponents: lentiviralexpression vector (e.g., pCDH-EF1-MCS-T2A-Puro) lentiviralpackaging plasmids (e.g., pPACKH1 Packaging Plasmid mix) pseudoviralparticle producer cell line (e.g., 293TN cells) 2A … SpletpLVX-TetOne-Puro载体质粒图谱、序列、价格、抗性、测序引物、大小等基本信息_生物风载体 pLVX-TetOne-Puro 价格： 6500元 联系方式： I47-825O-882O 相关技术服务： 质粒构建 基因合成 质粒大提 立刻购买 买家导航 载体基本信息 载体质粒图谱和多克隆位点信息 载体简介 载体序列
Then plvx-tetone-3xf-n-term puro
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SpletProducing lentivirus with the Lenti-X HT Packaging System. A Lenti-X Vector (pLVX-Tight-Puro) and the Lenti-X HT Packaging Mix are cotransfected into 293T cells using the highly efficient Lentiphos HT transfection system. High titer supernatants are ready for use 48 hr after transfection. SpletThe Lenti-X Tet-One Inducible Expression System (Puro) is a tetracycline-inducible lentiviral gene expression system that allows you to produce high titers of recombinant, VSV-G pseudotyped lentiviruses for the purpose of establishing a tightly inducible expression system for your gene of interest in a wide variety of dividing and non-dividing …
http://www.youbio.cn/product/vt8006 Splet10. jun. 2024 · An artificially synthesized MCS-P PGK -TetON3G-P2A-Puro fragment was PCR-amplified with Pfu DNA Polymerase (Promega, Madison, WI, USA) and excised with Cla I, followed by ligation to the above recovered pLVX-Puro, generating pLVX-Tet2A-Puro.
SpletIn addition to lentiviral elements, pLVX-Puro contains a puromycin resistance gene (Puror) under the control of the murine phosphoglycerate kinase (PGK) promoter (P. PGK. ) for … Splet优宝生物提供pLVX-IRES-Puro-3xFlag载体,产品优质,服务快速,同时提供pLVX-IRES-Puro-3xFlag质粒图谱,载体序列,载体抗性及操作手册等信息
SpletpRetroX-TetOne-Puro Sequence and Map pRetroX-TetOne-Puro All-in-one retroviral vector with a puromycin resistance marker, for strong inducible expression of genes using the …
Splet21. jul. 2024 · pLVX-Puro慢病毒载体使用说明.pdf. 2024-07-21上传. pLVX-Puro慢病毒载体使用说明 gst opinionSplet27. maj 2024 · 载体描述. pLVX-IRES-Puro is an HIV-1-based, lentiviral expression vector that allows the simultaneous expression of your protein of interest and puromycin resistance (Puror ) in virtually any mammalian cell type, including primary cells. The vector expresses your protein of interest and Puror from a bicistronic mRNA transcript, allowing ... gstop isterminal directionSpletThe pLVX-TetOne-Puro Vector Set, which is part of the Lenti-X Tet-One Inducible Expression System \(Puro\) allows for the lentiviral delivery and inducible expression of your gene of … gstop church fort worthSplet16. sep. 2024 · NKTCLs are almost exclusively extranodal involving the nasal and/or paranasal area, with a strong association with Epstein–Barr virus (EBV) infection 3, 4, 6, 7, 8. Despite a multimodality... financial markets united states wikiSplet31. maj 2024 · The pMD2.G and psPAX2 packaging plasmids and the pLVX-TetOne-Puro vector constructs for expressing ZNF24 or β-catenin overexpression vector were transfected into HEK293 cells to silence expression using Lipofectamine 2000 (Cat.No.11668019, Invitrogen) in accordance with specific protocols. ... Then, we chosen … financial markets training londonSpletpLVX-TetOne Sequence and Map pLVX-TetOne All-in-one lentiviral vector for strong inducible expression of genes using the Tet-On® system. Sequence Author: Clontech … financial markets versus financial securitiesSpletThe all-in-one pLVX-TetOne-Puro Vector expresses the Tet-On 3G transactivator from the constitutive human PGK promoter in the forward orientation and your gene of interest … gst open account